Multiple cell and 3D systems are available to meet your specific needs:
2D genetically engineered skin cell models
SkinAxis provides intact and genetically engineered primary human skin models including epidermal keratinocytes, melanocytes, and dermal fibroblasts. Each cell type has been engineered to carry a stable transgene that allows the expression of the reporter upon activation of specific pathways. For testing of active ingredients, we follow the cellular response and reporter gene in cell-specific culture medium.
3D skin models
SkinAxis established a wide range of in vitro 3D skin models. We provide 3D models developed using different types of skin cells. SkinAxis’ novel approach is based on the co-culture of multiple human primary skin cells in three-dimensional (3D) systems in physiologically relevant conditions in which skin’s native biology can be reconstituted and analyzed. We establish air-liquid interface in 3D models to generate stratification and differentiation of skin cells.
These models can be used to identify and validate active ingredients or final products.
Genetically engineered 3D skin models
SkinAxis generates genetically engineered 3D skin models following the transduction of primary skin cells with viral vectors carrying reporter genes under the control of tissue specific promoters. SkinAxis has developed a series of vectors and cell lines for the expression of fluorescent as well as chemiluminescent reporter proteins. The engineered cells maintain their physiological responses while allowing the precise assessment and more detailed analysis of the regulation of skin molecular targets upon treatment with drugs and active ingredients.
3D skin models for detection of UV damage
SkinAxis generates 3D skin models for the highly sensitive detection of UV damage using genetically engineered skin cells. Each cell type is engineered to allow the expression a reporter gene upon activation of the DNA damage response pathway. As UV irradiation causes DNA damage, these cells are particularly suitable to assess the effects of UV on skin in a quantifiable manner. Engineered cells are able to reconstitute the normal architecture of skin tissue after establishing air-liquid interface and cultivation in differentiation media. Novel 3D skin models allow rapid and quantitative assessment of the effects of UVA and UVB irradiation.
3D skin models for anti-melanoma drug screening
SkinAxis provides multicellular 3D skin equivalent models that support the growth and progression of melanoma in a physiologically relevant tumor microenvironment. These models could be custom modified to provide testing platforms for specific inhibitors of melanoma growth including BRAF-inhibitors. These models will significantly impact the efficiency of preclinical studies by reducing the attrition rate of molecules under investigation.
Oral mucosa equivalent
SkinAxis oral mucosa equivalent contains multiple layers of non-differentiated cells that could be induced to differentiate and form a multistrata, polarized, stratified epithelial layer with superficial keratinization. These models can be used for drug toxicity testing and investigation of therapeutic mechanisms.
SkinAxis, LLC is a biotechnology company that develops advanced technologies for skin research for the pharmaceutical, chemical, and cosmetic industries involved in drug and active ingredient discovery.
SkinAxis relies on a unique technology and innovative know-how to develop novel 3D skin equivalent models. This technology provides a flexible, highly sensitive, and reproducible platform for investigation and drug discovery in normal and diseased skin under physiological conditions.